Journal: Translational Oncology

Article Title: Upregulation of miR-142-3p Improves Drug Sensitivity of Acute Myelogenous Leukemia through Reducing P-Glycoprotein and Repressing Autophagy by Targeting HMGB1

doi: 10.1016/j.tranon.2017.03.003

Figure Lengend Snippet: HMGB1 reversed miR-142-3p–induced drug sensitivity in HL-60/ATRA and HL-60/ADR cells. (A and B) After cells treated with ATRA or ADR were transfected with miR-142-3p, miR-control or along with pcDNA-HMGB1 or vector, cell viability was determined by MTT assay. (C and D) After cells treated with ATRA or ADR were transfected with miR-142-3p, miR-control or along with pcDNA-HMGB1 or vector, apoptosis was examined by flow cytometry. * P < .05.

Article Snippet: miR-142-3p mimics (miR-142-3p), anti–miR-142-3p, miRNA control (miR-control), pcDNA-HMGB1, and pcDNA empty control (vector) were purchased from Genepharma (Shanghai, China).

Techniques: Transfection, Plasmid Preparation, MTT Assay, Flow Cytometry

Journal: Translational Oncology

Article Title: Upregulation of miR-142-3p Improves Drug Sensitivity of Acute Myelogenous Leukemia through Reducing P-Glycoprotein and Repressing Autophagy by Targeting HMGB1

doi: 10.1016/j.tranon.2017.03.003

Figure Lengend Snippet: miR-142-3p upregulation enhanced drug sensitivity of AML cells through reducing P-gp and suppressing autophagy by targeting HMGB1. The level of P-gp in HL-60/ATRA (A) and HL-60/ADR (B) cells transfected with miR-142-3p or miR-control, or together with pcDNA-HMGB1 or vector were detected by Western blot. The levels of autophagy related proteins Atg5, LC3-I, and LC3-II were analyzed by Western blot in HL-60/ATRA (C) and HL-60/ADR (D) cells transfected with miR-142-3p or miR-control, or together with pcDNA-HMGB1 or vector. * P < .05.

Article Snippet: miR-142-3p mimics (miR-142-3p), anti–miR-142-3p, miRNA control (miR-control), pcDNA-HMGB1, and pcDNA empty control (vector) were purchased from Genepharma (Shanghai, China).

Techniques: Transfection, Plasmid Preparation, Western Blot

Journal: Molecular Medicine Reports

Article Title: circSAMD4A participates in the apoptosis and autophagy of dopaminergic neurons via the miR-29c-3p-mediated AMPK/mTOR pathway in Parkinson's disease

doi: 10.3892/mmr.2021.12179

Figure Lengend Snippet: circSAMD4A targets and negatively regulates miR-29c-3p. (A) Upper panel, TargetScan analysis showing the putative recognition sequence of miR-29c-3p in circSAMD4A. Lower panel, a dual-luciferase reporter was used to verify the interaction between circSAMD4A and miR-29c-3p in 293 cells. The relative expression levels of (B) circSAMD4A and (C) miR-29c-3p were detected in SH-SY5Y cells transfected with sh-circ, pcDNA-circSAMD4A or matched controls via reverse transcription-quantitative PCR. **P<0.01, ***P<0.001. sh-circ, sh-circSAMD4A; miR, microRNA; circSAMD4A, circular RNA sterile α motif domain containing 4A; WT, wild-type; MUT, mutant; sh, short hairpin RNA; NC, negative control.

Article Snippet: miR-29c-3p mimics (including miR control, cat. no. HMI0439, concentration: ≥10 6 TU/ml), miR-29c-3p inhibitor (including miR-29c-3p inhibitor NC, cat. no. HSTUD0440, concentration: ≥10 6 TU/ml) were purchased from Sigma-Aldrich (Shanghai) Trading Co., Ltd., shNC (cat. no. C01001, concentration: 10 8 TU/ml), sh-circ (cat. no. C03002, concentration: 10 8 TU/ml), pcDNA empty vector (cat. no. G01001, concentration: 10 8 TU/ml), and pcDNA-circSAMD4A (cat. no. G04002, concentration: 10 8 TU/ml) were purchased from Shanghai GenePharma Co., Ltd.

Techniques: Sequencing, Luciferase, Expressing, Transfection, Real-time Polymerase Chain Reaction, Mutagenesis, shRNA, Negative Control